Department of Pathology was established in year 1943. Teaching, diagnosis, research and extension activities are the main working areas of the department. The department has a rich museum and great collection of slides and specimens of varieties of disease conditions in animals.

Initially, the department was established to teach pathology, histology, and meat inspection. Dr. K. S. R. Iyer was the first Professor. He was succeeded by Dr. R. M. Kalapesi in 1952. Dr. B. L. Purohit became professor in 1956. On his promotion as Principal of Nagpur Veterinary College, Dr. S. M. Ajinkya took over in 1962 as Professor who was succeeded by Dr. P. D. Sardeshpande in 1970. Later, Dr. B. V. Jalnapurkar handled the department as a Professor followed by Dr. P. S. Lonkar (1994 to 2005); Dr. Mrs. R. S. Nehete (2005 to 2010), and Dr. S. D. Moregaonkar from 2012 to 2017.

All of them contributed notably to the development of the department. They were ably supported by Dr. K. V. Joshi, Dr. N. S. Deodhar, and Dr. B. D. Survashe, who worked in different capacities. Currently, Dr. D. P. Kadam is In-charge Professor of the Department.